UNVEILING ANTIFUNGAL PROTEINS FROM TRAMETES VERSICOLOR AS BIO-PESTICIDE TO INHIBIT ALTERNARIA SOLANI

Alternaria solani was isolated from infected tomato leaves, and Trametes versicolor northern areas of Pakistan established mycelial culture on PDA. Crude Protein extract prepared in 50mM Sodium phosphate buffer by macerating C. (T/C) mycelium. Extracted protein solution (Crude extract) filtered tested for antifungal activity using different concentrations (0.5mg/ml, 1mg/ml, 1.5mg/ml, 2mg/ml) against A. solani. A with potential purified the DEAE- Cellulose column followed gel filtration Superdex75. MIC also observed micro spectrophotometry. Results showed that T. has to inhibit fungal growth a concentration 2mg/mL inhibited 83% However, only 47% inhibition case 0.5mg/ml extract. The having anion exchange chromatography an approximate size 15 kDa. is 200µg, which 100%